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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/02/2012 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OSTERNO, J. J.; COSTA, A. P. da; LANDIM, A. V.; LIMA, F. R. G. de; ROGERIO, M. C. P.; VASCONCELOS, A. M. de. |
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Afiliação: |
Juliana Justino Osterno; Aparecido Porto da Costa; Aline Vieira Landim; Fátima Révia Granja de Lima; MARCOS CLAUDIO PINHEIRO ROGERIO, CNPC; Angela Maria de Vasconcelos. |
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Título: |
Atributos fisiológicos de caprinos leiteiros submetidos a diferentes fases de aleitamento. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: ENCONTRO DE PESQUISA E PÓS-GRADUAÇÃO DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 6., 2011, Sobral. [Anais...]. Sobral: UVA, 2011. 5 f. |
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Idioma: |
Português |
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Conteúdo: |
Verificar as respostas dos atributos fisiológicos de cabritos leiteiros em diferentes idades de desaleitamento criados em condições semiáridas. |
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Palavras-Chave: |
Ambiencia animal; Caprino leiteiro; Conforto térmico. |
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Thesagro: |
Cabrito; Homeotermia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54752/1/AAC-Atributos-fisiologicos.pdf
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Marc: |
LEADER 00930nam a2200229 a 4500
001 1916874
005 2019-09-23
008 2011 bl uuuu u00u1 u #d
100 1 $aOSTERNO, J. J.
245 $aAtributos fisiológicos de caprinos leiteiros submetidos a diferentes fases de aleitamento.$h[electronic resource]
260 $aIn: ENCONTRO DE PESQUISA E PÓS-GRADUAÇÃO DA UNIVERSIDADE ESTADUAL VALE DO ACARAÚ, 6., 2011, Sobral. [Anais...]. Sobral: UVA, 2011. 5 f.$c2011
520 $aVerificar as respostas dos atributos fisiológicos de cabritos leiteiros em diferentes idades de desaleitamento criados em condições semiáridas.
650 $aCabrito
650 $aHomeotermia
653 $aAmbiencia animal
653 $aCaprino leiteiro
653 $aConforto térmico
700 1 $aCOSTA, A. P. da
700 1 $aLANDIM, A. V.
700 1 $aLIMA, F. R. G. de
700 1 $aROGERIO, M. C. P.
700 1 $aVASCONCELOS, A. M. de
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido; Embrapa Uva e Vinho. |
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Data corrente: |
24/02/2022 |
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Data da última atualização: |
14/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
WAIRICH, A.; MALABARBA, J.; BUFFON, V.; PORTO, D. D.; TOGAWA, R. C.; REVERS, L. F. |
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Afiliação: |
ANDRIELE WAIRICH, Universidade Federal do Rio Grande do Sul (UFRGS); JAIANA MALABARBA, CNPUV; VANESSA BUFFON, CNPUV; DIOGO DENARDI PORTO, CPATSA; ROBERTO COITI TOGAWA, Cenargen; LUIS FERNANDO REVERS, CNPUV. |
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Título: |
Molecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Euphytica, v. 218, 2022. Article, 5. |
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DOI: |
https://doi.org/10.1007/s10681-021-02952-3 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Roberto Togawa. |
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Conteúdo: |
Plasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance against P. viticola infection and presents promising biotechnological tools for the selection of young resistant individuals. MenosPlasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance agai... Mostrar Tudo |
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Palavras-Chave: |
Downy mildew resistance; Resistência ao míldio; Resistência ao oídio; Rpv3 locus; RT-qPCR; V vinifera. |
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Thesagro: |
Doença; Míldio; Uva; Vitis Vinifera. |
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Thesaurus NAL: |
Grapes; Marker-assisted selection; Plasmopara viticola. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232084/1/Wairich2021-Article-MolecularCharacterizationOfThe.pdf
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Marc: |
LEADER 02696naa a2200361 a 4500
001 2140494
005 2022-07-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10681-021-02952-3$2DOI
100 1 $aWAIRICH, A.
245 $aMolecular characterization of the Rpv3 locus towards the development of KASP markers for downy mildew resistance in grapevine (Vitis spp.).$h[electronic resource]
260 $c2022
500 $aNa publicação: Roberto Togawa.
520 $aPlasmopara viticola is the oomycete that causes downy mildew in grapevines. Varying levels of resistance to P. viticola across grape cultivars allowed quantitative trait loci to be identified. The Rpv3 locus is located at chromosome 18, in a region enriched in TIR-NBS-LRR genes, and the phenotype associated is a high hypersensitive response. In this work, we aimed to identify candidate genes associated with resistance to downy mildew on the Rpv3 locus and to evaluate their transcriptional profiles in a susceptible and a resistant grapevine cultivar after challenging with P. viticola. Candidate genes were identified by in silico functional enrichment tests. Many predicted genes associated with resistance to diseases were found at the Rpv3 locus. In total, seventeen genes were evaluated by RT-qPCR. Differences in the steady-state expression of these genes were observed between the two cultivars. Four genes were found to be expressed only in Villard Blanc, suggesting their association to the hypersensitivity reaction. Aiming to assist marker assisted-selection for downy mildew resistance, we show the efficient use of a set of SSR markers. Furthermore, from on a set of forty-one Rpv3-located SNPs, whose segregation was tested in the populations studied, the two segregating markers, Rpv3_15 and Rpv3_33, were considered efficient for downy mildew resistance identification. This study presents a genomic characterization of the Rpv3 locus, confirms its involvement in resistance against P. viticola infection and presents promising biotechnological tools for the selection of young resistant individuals.
650 $aGrapes
650 $aMarker-assisted selection
650 $aPlasmopara viticola
650 $aDoença
650 $aMíldio
650 $aUva
650 $aVitis Vinifera
653 $aDowny mildew resistance
653 $aResistência ao míldio
653 $aResistência ao oídio
653 $aRpv3 locus
653 $aRT-qPCR
653 $aV vinifera
700 1 $aMALABARBA, J.
700 1 $aBUFFON, V.
700 1 $aPORTO, D. D.
700 1 $aTOGAWA, R. C.
700 1 $aREVERS, L. F.
773 $tEuphytica$gv. 218, 2022. Article, 5.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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